Postdoctoral Scholar - Cardiology
Position Overview
Organization: Medicine, Cardiology
Title: Postdoctoral Scholar - Cardiology
Position Details
Position Description
A postdoctoral position is available in the Division of Cardiology, Department of Medicine at the University of Washington to study the role of mitochondria-containing extracellular vesicles (MitoEV) in sterile inflammation associated with heart failure (HF) and type 2 diabetes (T2D).
This is a full-time Postdoctoral Scholar position on an annual, 12-month appointment. Postdoctoral scholars are represented by UAW 4121 and are subject to the collective bargaining agreement, unless agreed exclusion criteria apply. For more information, please visit the University of Washington Labor Relations website.
The project pursues investigations in the immune-modulating roles of circulating MitoEV in sterile inflammation in chronic conditions such as HF and T2D. Specifically, studies are carried out to study the regulation and mechanism of MitoEV release from peripheral immune cells in the setting of chronic inflammation, and the tissue-specific targets of proinflammatory MitoEV. Approaches include live-cell confocal and electron microscopy, flow analysis, mRNA/protein analyses, and genetic modifications by CRISPR-knock using cell-based and animal models. Experience in confocal microscopy and mouse models is highly desirable.
- Design and perform cell-based experiments (50%):
-
- Generation of gene-deletions monocyte line(s) by CRISPR
- Volume-metric Flow analysis to quantify released MitoEV
- Confocal and electron microscopy of subcellular structures (e.g. mitochondria-containing endosomes)
- Functional and anatomical assessment of mitochondria and lysosome using biochemical assays
- Functional assessment of mitophagy/autophagy by live-cell imaging and flow analysis
- Develop mouse models of MitoEV-induced inflammation (50%):
- Generate gene-knock out mouse line(s) and maintain mouse colonies.
- Venous injection of isolated MitoEV
- Small animal nuclear imaging studies to determine tissue tropism of injected MitoEV
- Tissue analysis of inflammation
- Trans-thoracic echocardiogram to determine LV functions
The University of Washington is a rich environment for research in cardiovascular biology and disease. The Pacific Northwest is a pleasant place to live. Competitive salary. Opportunities for post-doc level grants, such as T32, F32, and AHA post-doc fellowship.
The base salary range for this position will be $5,705 per month, commensurate with experience and qualifications, or as mandated by a U.S. Department of Labor prevailing wage determination.
Qualifications
The successful candidate must have a Ph.D. or equivalent degree relevant to biological science and a strong publication record, particularly first-authorships. Prefer applicants with predilection toward an academic career.
Instructions
Please submit a cover letter and CV as well as the names/emails for three references through Interfolio. With questions, you may contact Dennis Wang, MD, PhD, Division of Cardiology, University of Washington, ddwang2@uw.edu
Equal Employment Opportunity Statement
University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, creed, religion, national origin, sex, sexual orientation, marital status, pregnancy, genetic information, gender identity or expression, age, disability, or protected veteran status.
Benefits Information
A summary of benefits associated with this title/rank can be found at https://hr.uw.edu/benefits/benefits-orientation/benefit-summary-pdfs/. Appointees solely employed and paid directly by a non-UW entity are not UW employees and are not eligible for UW or Washington State employee benefits.
Commitment to Diversity
The University of Washington is committed to building diversity among its faculty, librarian, staff, and student communities, and articulates that commitment in the UW Diversity Blueprint (http://www.washington.edu/diversity/diversity-blueprint/). Additionally, the University’s Faculty Code recognizes faculty efforts in research, teaching and/or service that address diversity and equal opportunity as important contributions to a faculty member’s academic profile and responsibilities (https://www.washington.edu/admin/rules/policies/FCG/FCCH24.html#2432).
Privacy Notice
Review the University of Washington Privacy Notice for Demographic Data of Job Applicants and University Personnel to learn how your demographic data are protected, when the data may be used, and your rights.
Disability Services
To request disability accommodation in the application process, contact the Disability Services Office at 206-543-6450 or dso@uw.edu.